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- MACHEREY-NAGEL 720050.46 Nucleosil 120-5 C8 H...
MACHEREY-NAGEL 720050.46 Nucleosil 120-5 C8 HPLC 1 Column, Analytical, 125 mm Length, 4.6 mm ID, USP L7
XCD 2203
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NUCLEOSIL: the trusted choice for versatile chromatography with unparalleled purity and uniformity.
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Product Details
| Item Weight | 0.3 lbs (140 grams) |
Who Should Buy?
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Analytical Chemists
Ideal for analytical chemists specializing in HPLC method development and optimization in various laboratories.
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Pharmaceutical Researchers
Useful for pharmaceutical researchers conducting drug analysis and formulation studies to ensure product efficacy.
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Academic Institutions
Recommended for academic institutions where students and researchers explore chromatography techniques in scientific studies.
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Beginners
Not suitable for beginners without prior knowledge of HPLC methods and chromatographic techniques.
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Non-HPLC Users
Not advised for users who do not utilize HPLC systems for their analytical or research needs.
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Budget-Conscious
May not fit users with tight budgets since it represents a higher investment for chromatography equipment.
Product Description
MACHEREY-NAGEL 720050.46 Nucleosil 120-5 C8 HPLC 1 Column, Analytical, 125 mm Length, 4.6 mm ID, USP L7
Customer Questions & Answers
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Question:
What is the specification of the MACHEREY-NAGEL 720050.46 Nucleosil 120-5 C8 HPLC column?
Answer: The MACHEREY-NAGEL 720050.46 Nucleosil 120-5 C8 HPLC column features an analytical design with a length of 125 mm and an internal diameter (ID) of 4.6 mm. It is packed with C8 bonded phases, allowing for effective separation of various compounds. This column is categorized under USP L7, suitable for diverse applications in analytical HPLC. Its specific dimensions provide optimal flow rates and sample retention times, making it ideal for labs focused on precision and reproducibility in their chemical analysis. -
Question:
What applications can I use the MACHEREY-NAGEL Nucleosil 120-5 C8 column for?
Answer: The MACHEREY-NAGEL Nucleosil 120-5 C8 column is versatile and can be used in various applications such as pharmaceutical analysis, environmental testing, and food quality assessment. Its C8 phase allows for good retention of mid-polar compounds, making it ideal for separating complex mixtures effectively. Whether you need to analyze drug compounds, pesticide residues, or natural products, this column can provide reliable and efficient results in your HPLC system. -
Question:
How does the Nucleosil 120-5 C8 column compare to other columns?
Answer: Compared to other HPLC columns, the Nucleosil 120-5 C8 stands out due to its unique C8 bonded phase that offers a balance between polarity and hydrophobic interaction. This allows for enhanced separation efficiency and resolution of complex mixtures. Unlike traditional C18 columns, the C8 phase provides shorter retention times, making it particularly useful for analyzing samples that are sensitive to prolonged interactions, ensuring you can achieve precise results quickly. -
Question:
What type of solvents are compatible with MACHEREY-NAGEL Nucleosil 120-5 C8?
Answer: The MACHEREY-NAGEL Nucleosil 120-5 C8 column is compatible with a range of solvents including acetonitrile, methanol, and water-based mobile phases. When developing your method, it’s essential to choose solvents that match the polarity of your analytes. This compatibility ensures smooth flow and optimal interaction, allowing for effective separation. Always consult your method documentation to tailor your solvent choice to your specific analytical needs. -
Question:
What is the particle size of the Nucleosil 120-5 C8 column, and how does it affect performance?
Answer: The MACHEREY-NAGEL Nucleosil 120-5 C8 column features a particle size of 5 micrometers. This size contributes to a high surface area, enhancing the interaction between the analytes and the stationary phase. Smaller particle sizes generally lead to better separation efficiency and resolution, allowing for the detection of closely eluting compounds. In practice, this means that for complex mixtures, you can expect improved peak shape and clarity in your chromatograms. -
Question:
Can I use the Nucleosil 120-5 C8 column at high temperatures?
Answer: Yes, the MACHEREY-NAGEL Nucleosil 120-5 C8 column can be utilized at elevated temperatures, typically up to 60 degrees Celsius, depending on the compatibility of your solvents. High-temperature operation can reduce viscosity and enhance mass transfer, leading to shorter run times and sharper peaks. However, it's crucial to consult specific protocols or guidelines to ensure that the column's integrity and performance are maintained under higher temperatures. -
Question:
Is the MACHEREY-NAGEL Nucleosil C8 suitable for analysis of large biomolecules?
Answer: The MACHEREY-NAGEL Nucleosil 120-5 C8 column is primarily designed for small to mid-sized organic molecules. While it may not be the best choice for large biomolecules such as proteins or nucleic acids due to steric hindrance, it excels with small polar and non-polar compounds. For biomolecular applications, consider using dedicated columns designed for bioanalysis which provide the necessary surface chemistry to accommodate larger analytes. -
Question:
How do I properly store the Nucleosil 120-5 C8 column?
Answer: To ensure the longevity and performance of your MACHEREY-NAGEL Nucleosil 120-5 C8 column, store it in a suitable mobile phase, ideally a mixture of water and organic solvents, to prevent drying and contamination. Maintain the column in a cool, dark environment to avoid exposure to extreme temperatures and UV light. Proper storage will help maintain the integrity of the stationary phase and ensure consistent performance in your applications. -
Question:
What is the lifespan of the MACHEREY-NAGEL Nucleosil 120-5 C8 column?
Answer: The lifespan of the MACHEREY-NAGEL Nucleosil 120-5 C8 column can vary significantly based on usage conditions, sample composition, and maintenance practices. Generally, if used under optimal conditions with regular cleaning and proper storage, you can achieve a lifespan of several hundred to a few thousand injections. Keeping an eye on peak shape and retention times can help you assess when it's time for a replacement. -
Question:
Where can I buy MACHEREY-NAGEL 720050.46 Nucleosil 120-5 C8 HPLC column?
Answer: You can purchase the MACHEREY-NAGEL 720050.46 Nucleosil 120-5 C8 HPLC column from Ubuy. Ubuy is a reliable e-commerce platform that offers a wide selection of laboratory equipment and supplies, ensuring you can find authentic and quality products to meet your research needs in Saint Lucia.
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XCD 2203
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Features & Benefits
- Totally porous spherical silicas designed for optimal performance.
- Endcapped and featuring a pore size of 100 Å and particle size of 5 µm.
- Separation mechanism based on hydrophobic interactions for effective analysis.
- Highly accepted in various fields of chromatography.
- Pioneering choice in High-Performance Liquid Chromatography (HPLC).
- Delivers reliable results with its pure SiO2 structure.