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Item #:
83401258
1X TE Buffer, pH 7.6 - Molecular Biology Grade - RNase/DNase Free, Sterile, 0.22um Filtered - 500mL
88% of respondents would recommend this to a friend
Item #:
83401258
XCD 107
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Our TE Buffer is RNase/DNase free, sterile and 0.22um filtered ensuring premium quality.
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What Stands Out
High Purity
This TE buffer is RNase and DNase free, ensuring that your samples remain uncontaminated for accurate results, making it ideal for sensitive molecular biology applications.
Sterile Packaging
Provided in a sterile, 0.22μm filtered format, this buffer guarantees that any microbial contamination is eliminated, preserving the integrity of your experiments.
Optimal pH
With a precise pH of 7.6, this TE buffer is specially formulated to maintain the stability of nucleic acids, enhancing overall experimental reliability compared to competitors.
Product Details
| Brand Name | UFC Bio |
| Manufacturer | UFC Bio |
| UPC | 608037807656 |
| Part Number | BTE-1X74 |
| Included Components | Molecular Biology Grade 1X TE Buffer, pH 7.4 |
| Sterility Rating | 7.6 |
| Recommended Uses For Product | Nucleic Acid Purification, Electrophoresis, Nucleic Acid Storage |
| Brand | UFC Bio |
| Ph Range | 7.6 |
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Molecular Biologists
Essential for experiments where DNA/RNA purification is required, ensuring minimal contamination and optimal results.
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Laboratory Technicians
Ideal for routine lab work that involves nucleic acid storage and manipulation, providing reliable reagents.
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Educational Institutions
Useful for teaching purposes in molecular biology courses, allowing students to work with high-quality buffers.
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General Use
Not suitable for applications outside molecular biology requiring different buffer compositions or concentrations.
Product Description
1X TE Buffer, pH 7.6 - Molecular Biology Grade - RNase/DNase Free, Sterile, 0.22um Filtered - 500mL
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Customer Questions & Answers
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Question:
What is TE Buffer used for in molecular biology?
Answer: TE Buffer is commonly used to maintain the stability of nucleic acids during storage and various molecular biology procedures. It helps preserve DNA or RNA by providing an optimal pH environment, typically at 7.6, which minimizes degradation. Researchers often utilize TE Buffer for dilutions, enzyme reactions, or storing plasmids and genomic DNA, as it ensures the integrity of samples over time. -
Question:
Is the 1X TE Buffer RNase/DNase free?
Answer: Yes, the 1X TE Buffer is specifically formulated to be RNase and DNase free, making it suitable for sensitive molecular biology applications. This lack of nucleases means that your nucleic acids remain intact and uncontaminated, preserving their integrity for downstream applications. This is particularly crucial when working with precious samples where contamination could lead to undesirable results. -
Question:
Why is pH 7.6 important in TE Buffer?
Answer: A pH of 7.6 for TE Buffer ensures a balanced and stable environment for nucleic acids, which is essential for optimal performance during various molecular biology techniques. This slightly alkaline pH helps to prevent hydrolysis of nucleotides and maintains the solubility of the buffer components, enhancing the overall efficacy of nucleic acid handling. It is especially important when preparing samples for PCR or cloning. -
Question:
What does 'sterile' mean in relation to TE Buffer?
Answer: The term 'sterile' indicates that the TE Buffer has been processed to eliminate any microorganisms, ensuring it is free from biological contaminants. This sterility is crucial for maintaining the integrity of your molecular biology experiments, as any contamination could lead to skewed results or sample degradation. For instance, researchers use sterile TE Buffer for cloning procedures to prevent unwanted microbial growth. -
Question:
What does '0.22 um filtered' mean, and why is it necessary?
Answer: ‘0.22 um filtered’ signifies that the TE Buffer has been passed through a filter that removes particles larger than 0.22 micrometers. This filtration process is essential to eliminate any particulates or microorganisms that could interfere with molecular biology experiments. Such filtration is particularly important when preparing reagents for high-precision applications like qPCR or sequencing. -
Question:
Can I use TE Buffer for storing RNA?
Answer: Yes, TE Buffer is suitable for storing RNA, as its RNase-free formulation effectively protects RNA integrity over time. When stored in TE Buffer at the recommended pH, RNA samples remain stable and are less susceptible to degradation. Researchers typically employ TE Buffer to preserve RNA extracted from tissues for downstream applications like RT-PCR. -
Question:
How should TE Buffer be stored for optimal use?
Answer: TE Buffer should be stored at 2 to 8 degrees Celsius, away from light, to maintain its stability and effectiveness. This specific storage condition helps prevent any potential degradation of buffer components and ensures long-lasting performance. For active labs, aliquoting the buffer into smaller volumes can prevent repeated freeze-thaw cycles, preserving its integrity for all molecular biology applications. -
Question:
What is the volume of the TE Buffer product, and how is it typically used?
Answer: This TE Buffer is provided in a convenient 500mL volume, making it suitable for multiple applications in molecular biology. Its large size allows researchers to perform numerous experiments or store several samples without the need for frequent reordering. It's commonly used in various procedures such as DNA extraction, cloning, and routine maintenance of nucleic acid quality. -
Question:
Can TE Buffer be used with other buffers or solutions?
Answer: TE Buffer can be compatible with various other buffers or solutions, but caution is needed. Combining buffers may alter pH or ionic strength, potentially affecting subsequent reactions. It's often advisable to use TE Buffer in conjunction with protocols that specify its use to ensure optimal results in applications such as sequencing or PCR. Always consult specific protocols for guidance on compatibility. -
Question:
Where can I buy 1X TE Buffer, pH 7.6 in Saint Lucia?
Answer: You can purchase 1X TE Buffer, pH 7.6 through Ubuy, which offers a range of molecular biology products, including this specific buffer. Ubuy provides a reliable online platform where you can find various laboratory supplies tailored for your research needs. Explore their inventory to ensure you have the right tools for your experiments.
UFC Bio Buffers Editorial Review
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Pros
- High quality, sterile buffer
- RNase/DNase free
- Accurate pH level
- Reliable for molecular biology
- 500mL offers great value
Cons
- The bottle can be a bit bulky.
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Features & Benefits
- RNase/DNase free, sterile and 0.22um filtered TE Buffer
- Manufactured in USA with strict quality control
- 500mL size, room temperature storage
- For research use only - 1X solution, 10mM Tris, 1mM EDTA
- Expiration Date: 11/2023
- Product of Buffalo, NY
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